Laboratory/Bench

Description

Hands on laboratory bench work and expertise can be provided on a contract basis for any time frame.  Expert skill is available in the following areas:

Protein/Enzyme

  1. Purification
    • Fermentation
    • Extraction/Fractionation
    • Chromatography
      • Size Exclusion
      • Ion Exchange
      • Hydrophobic Interaction
  2. Enzyme Assay (specializing in Oxygenases/Oxidases)
    • Spectrophotometric
    • Fluorometric
    • Chemiluminescent
    • Calorimetric
    • Radiometric (32P, 35S)
  3. Enzyme Kinetics
    • Steady State
    • Pre-steady State

Nucleic Acids

  1. Extraction & Purification
  2. Southerns & Northerns
  3. Site Directed Mutagenesis
  4. Recombinant Protein Expression/Purification
    • Cloning
    • Host/Promoter optimization
    • Western Blotting
    • Fermentation/Expression Optimization
  5. Electrophoretic Mobility Shift Assay (EMSA)
  6. Chemical Based Protein/Nucleic Acid structural determination